Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.].

ABSTRACT:

Background

To explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2^ry) and tertiary (3^ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2^ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1^ry lines initiated from zygotic embryos (SD4, 2155 vs 477; TD17, 240 vs 29 g^- 1 f.w.). Moreover, we observed similar differences in yield between 2^ry and 3^ry lines of SD4 (2400 vs 3921 g^- 1 f.w.). To elucidate reasons for differences in embryogenic potential induced by repetitive somatic embryogenesis we then compared 2^ry vs 1^ry and 2<

SUBMITTER: Gautier F 

PROVIDER: S-EPMC6086078 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature