Inhibition of Pseudomonas aeruginosa secreted virulence factors reduces lung inflammation in CF mice.

ABSTRACT:

Background

Cystic fibrosis (CF) lung infection is a complex condition where opportunistic pathogens and defective immune system cooperate in developing a constant cycle of infection and inflammation. The major pathogen, Pseudomonas aeruginosa, secretes a multitude of virulence factors involved in host immune response and lung tissue damage. In this study, we examined the possible anti-inflammatory effects of molecules inhibiting P. aeruginosa virulence factors.

Methods

Pyocyanin, pyoverdine and proteases were measured in bacterial culture supernatant from different P. aeruginosa strains. Inhibition of virulence factors by sub-inhibitory concentrations of clarithromycin and by protease inhibitors was evaluated. Lung inflammatory response was monitored by in vivo bioluminescen

SUBMITTER: Sandri A 

PROVIDER: S-EPMC6086295 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature