Characterization of recombinant wild-type and nontoxigenic protein A from Staphylococcus pseudintermedius.

ABSTRACT:

Background

Staphylococcus pseudintermedius is an opportunistic pathogen that is the major cause of pyoderma affecting dogs. Conventional antimicrobial treatment for infections caused by this organism have failed in recent years due to widespread resistance and alternative treatment strategies are a high priority. Protein A encoded in Staphylococcus aureus by spa protects the bacterium by binding IgG and acts as a superantigen. Staphylococcus pseudintermedius possess two genes orthologous to S. aureus spa, spsP, and spsQ.

Methods

SpsQ and SpsQ-M, a non-toxigenic SpsQ, were cloned and expressed as recombinant proteins and their cytotoxic effect on canine B cells was measured. The neutralizing ability of antibody raised against them in clinically healthy dogs was evaluated.

SUBMITTER: Abouelkhair MA 

PROVIDER: S-EPMC6086296 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature