Geometry processing of conventionally produced mouse brain slice images.

ABSTRACT: BACKGROUND:Brain mapping research in most neuroanatomical laboratories relies on conventional processing techniques, which often introduce histological artifacts such as tissue tears and tissue loss. NEW METHOD:In this paper, we present techniques and algorithms for automatic registration and 3D reconstruction of conventionally produced mouse brain slices in a standardized atlas space. This is achieved first by constructing a virtual 3D mouse brain model from annotated slices of Allen Reference Atlas (ARA). Virtual re-slicing of the reconstructed model generates ARA-based slice images corresponding to the microscopic images of histological brain sections. These image pairs are aligned using a geometric approach through contour images. Histological artifacts in the microscopic images are de

SUBMITTER: Agarwal N 

PROVIDER: S-EPMC6086593 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature