Assessment of developmental potential of human single pronucleated zygotes derived from conventional in vitro fertilization.

ABSTRACT:

Purpose

The aim of this study was to non-invasively validate the developmental potential of human single pronucleated (1PN) zygotes derived from conventional in vitro fertilization (c-IVF) at the zygote stage.

Methods

Fifty 1PN zygotes derived from 45 patients undergoing c-IVF were used. Immunohistochemistry and fluorescence live cell imaging were used to confirm normal chromosome segregation during the first mitosis. The usefulness of measuring pronuclear diameter was assessed on the basis of the presence or absence of a proper first cleavage and validated by subsequent development.

Results

Although approximately 80% (15/19) of 1PN zygotes contained a diploid genome, immunohistochemistry revealed an unequal distribution of paternal and maternal genomes at the first m

SUBMITTER: Kai Y 

PROVIDER: S-EPMC6086793 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature