Effect of patterned polyacrylamide hydrogel on morphology and orientation of cultured NRVMs.

ABSTRACT: We recently demonstrated that patterned Parylene C films could be effectively used as a mask for directly copolymerizing proteins on polyacrylamide hydrogel (PAm). In this work, we have proved the applicability of this technique for studying the effect such platforms render on neonatal rat ventricular myocytes (NRVMs). Firstly, we have characterised topographically and mechanically the scaffolds in liquid at the nano-scale level. We thus establish that such platforms have physical properties that closely mimics the in vivo extracellular environment of cells. We have then studied the cell morphology and physiology by comparing cultures on flat uniformly-covered and collagen-patterned scaffolds. We show that micro-patterns promote the elongation of cells along the principal axis of the ridge

SUBMITTER: Sanzari I 

PROVIDER: S-EPMC6086831 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature