Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation.

ABSTRACT: In the stem cell research field, the molecular regulatory network used to define cellular states has been extensively studied, however, the general driving force guiding the collective state dynamics remains to be identified from biophysical aspects. Here we monitored the time-development of the cell-state transition at the single-cell and colony levels, simultaneously, during the early differentiation process in mouse embryonic stem cells. Our quantitative analyses revealed that cellular heterogeneity was a result of spontaneous fluctuation of cellular state and cell-cell cooperativity. We considered that the cell state is like a ball fluctuating on a potential landscape, and found that the cooperativity affects the fluctuation. Importantly, the cooperativity temporarily decreased and inc

SUBMITTER: Okamoto K 

PROVIDER: S-EPMC6086879 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature