Statistical modelling for optimized lyophilization of Lactobacillus acidophilus strains for improved viability and stability using response surface methodology.

ABSTRACT: The awareness regarding use of probiotics for health benefits has created a huge market worldwide. The use of probiotic cultures and its success in commercial scale is relying on the techniques employed to ensure that the potential cultures with desired functional activity are stable and viable for longer period. The present study was undertaken to statistically evaluate the effect of lyoprotectant in the suspension media containing sucrose and reconstituted skim milk on the viability of Lactobacillus acidophilus strains. The central composite rotatable design of response surface methodology was used in evaluating the survival rate and viability even after 24 months' storage of three different lyophilized strains of L. acidophilus. We also determined the exclusive use of lyoprotectant on t

SUBMITTER: Turuvekere Sadguruprasad L 

PROVIDER: S-EPMC6086920 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature