Directed-Backbone Dissociation Following Bond-Specific Carbon-Sulfur UVPD at 213 nm.

ABSTRACT: Ultraviolet photodissociation or UVPD is an increasingly popular option for tandem-mass spectrometry experiments. UVPD can be carried out at many wavelengths, and it is important to understand how the results will be impacted by this choice. Here, we explore the utility of 213 nm photons for initiating bond-selective fragmentation. It is found that bonds previously determined to be labile at 266 nm, including carbon-iodine and sulfur-sulfur bonds, can also be cleaved with high selectivity at 213 nm. In addition, many carbon-sulfur bonds that are not subject to direct dissociation at 266 nm can be selectively fragmented at 213 nm. This capability can be used to site-specifically create alaninyl radicals that direct backbone dissociation at the radical site, creating diagnostic d-ions. Furth

SUBMITTER: Talbert LE 

PROVIDER: S-EPMC6087500 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature