Mycoflora isolation and molecular characterization of Aspergillus and Fusarium species in Tunisian cereals.

ABSTRACT: Wheat, barley and maize are the mainly consumed cereals in Tunisia. This study aimed to determine the mycoflora of these cereals with special focus on the mycotoxigenic Aspergillus and Fusarium species. Freshly harvested samples and other stored samples of each type of cereal (31 and 34 samples, respectively) were collected in Tunisia and cultured for fungal isolation and identification. Identification of fungal genera was based on morphological features. Aspergillus and Fusarium species were identified by species specific PCR assays complemented with DNA sequencing. Alternaria (70.83%), Eurotium (62.50%), Aspergillus (54.17%) and Penicillium (41.67%) were the most frequent fungi isolated from wheat. Penicillium (75%), Asper

SUBMITTER: Jedidi I 

PROVIDER: S-EPMC6088110 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature