Rapid access to RNA resonances by proton-detected solid-state NMR at >100 kHz MAS.

ABSTRACT: Fast (>100 kHz) magic angle spinning solid-state NMR allows combining high-sensitive proton detection with the absence of an intrinsic molecular weight limit. Using this technique we observe for the first time narrow ¹H RNA resonances and assign nucleotide spin systems with only 200 μg of uniformly ¹³C,¹⁵N-labelled RNA.

SUBMITTER: Marchanka A 

PROVIDER: S-EPMC6088370 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature