Data-independent acquisition-based SWATH-MS for quantitative proteomics: a tutorial.

ABSTRACT: Many research questions in fields such as personalized medicine, drug screens or systems biology depend on obtaining consistent and quantitatively accurate proteomics data from many samples. SWATH-MS is a specific variant of data-independent acquisition (DIA) methods and is emerging as a technology that combines deep proteome coverage capabilities with quantitative consistency and accuracy. In a SWATH-MS measurement, all ionized peptides of a given sample that fall within a specified mass range are fragmented in a systematic and unbiased fashion using rather large precursor isolation windows. To analyse SWATH-MS data, a strategy based on peptide-centric scoring has been established, which typically requires prior knowledge about the chromatographic and mass spectrometric behaviour of pepti

SUBMITTER: Ludwig C 

PROVIDER: S-EPMC6088389 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature