Stem-like and highly invasive prostate cancer cells expressing CD44v8-10 marker originate from CD44-negative cells.

ABSTRACT: In human prostate cancer (PCa), the neuroendocrine cells, expressing the prostate cancer stem cell (CSC) marker CD44, may be resistant to androgen ablation and promote tumor recurrence. During the study of heterogeneity of the highly aggressive neuroendocrine PCa cell lines PC3 and DU-145, we isolated and expanded in vitro a minor subpopulation of very small cells lacking CD44 (CD44^neg). Unexpectedly, these sorted CD44^neg cells rapidly and spontaneously converted to a stable CD44^high phenotype specifically expressing the CD44v8-10 isoform which the sorted CD44^high subpopulation failed to express. Surprisingly and potentially interesting, in these cells expression of CD44v8-10 was found to be induced in stem cell medium. CD44 variant isofor

SUBMITTER: Di Stefano C 

PROVIDER: S-EPMC6089404 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature