Stim and Orai mediate constitutive Ca²⁺ entry and control endoplasmic reticulum Ca²⁺ refilling in primary cultures of colorectal carcinoma cells.

ABSTRACT: Store-operated Ca²⁺ entry (SOCE) provides a major Ca²⁺ entry route in cancer cells. SOCE is mediated by the assembly of Stim and Orai proteins at endoplasmic reticulum (ER)-plasma membrane junctions upon depletion of the ER Ca²⁺ store. Additionally, Stim and Orai proteins underpin constitutive Ca²⁺ entry in a growing number of cancer cell types due to the partial depletion of their ER Ca²⁺ reservoir. Herein, we investigated for the first time the structure and function of SOCE in primary cultures of colorectal carcinoma (CRC) established from primary tumor (pCRC) and metastatic lesions (mCRC) of human subjects. Stim1-2 and Orai1-3 transcripts were equally expressed in pCRC and mCRC cells, although Stim1 and Orai3 proteins were up-regul

SUBMITTER: Zuccolo E 

PROVIDER: S-EPMC6089563 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature