A method for treatment monitoring using circulating tumour DNA in cancer patients without targetable mutations.

ABSTRACT:

Background

The potentials of circulating tumour DNA (ctDNA) have been studied for non-invasive disease monitoring in patients with targetable mutations. However, the majority of cancer patients harbour no targetable mutations. A workflow including targeted next-generation sequencing (NGS) and droplet digital PCR (ddPCR) could be used for monitoring treatment in these patients. Thus, our aim was to evaluate the workflow for ctDNA monitoring in a cohort of non-small cell lung cancer patients.

Methods

Forty patients were prospectively included. Plasma samples were collected prior to and during treatment. NGS (Ion AmpliSeq Colon and Lung Cancer panel v2) was performed on ctDNA from pre-treatment samples. The identified mutations were monitored by ddPCR in consecutively collected

SUBMITTER: Demuth C 

PROVIDER: S-EPMC6089565 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature