Visual and modular detection of pathogen nucleic acids with enzyme-DNA molecular complexes.

ABSTRACT: Rapid, visual detection of pathogen nucleic acids has broad applications in infection management. Here we present a modular detection platform, termed enzyme-assisted nanocomplexes for visual identification of nucleic acids (enVision). The system consists of an integrated circuit of enzyme-DNA nanostructures, which function as independent recognition and signaling elements, for direct and versatile detection of pathogen nucleic acids from infected cells. The built-in enzymatic cascades produce a rapid color readout for the naked eye; the assay is thus fast (<2 h), sensitive (<10 amol), and readily quantified with smartphones. When implemented on a configurable microfluidic platform, the technology demonstrates superior programmability to perform versatile computations, for detecting divers

SUBMITTER: Ho NRY 

PROVIDER: S-EPMC6089962 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature