Low Oxygen Levels Induce Early Luteinization Associated Changes in Bovine Granulosa Cells.

ABSTRACT: During follicle maturation, oxygen levels continuously decrease in the follicular fluid and reach lowest levels in the preovulatory follicle. The current study was designed to comprehensively understand effects of low oxygen levels on bovine granulosa cells (GC) using our established estrogen active GC culture model. As evident from flow cytometry analysis the viability of GC was not found to be affected at severely low oxygen condition (1% O₂) compared to normal (atmospheric) oxygen condition (21% O₂). Estimations of hormone concentrations using competitive radioimmunoassay revealed that the production of estradiol and progesterone was significantly reduced at low oxygen condition. To understand the genome-wide changes of gene expression, mRNA microarray analysis was

SUBMITTER: Baddela VS 

PROVIDER: S-EPMC6090175 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature