Functional Integrity and Gene Expression Profiles of Human Cord Blood-Derived Hematopoietic Stem and Progenitor Cells Generated In Vitro.

ABSTRACT: To date, different experimental strategies have been developed for the ex vivo expansion of human hematopoietic stem (HSCs) and progenitor (HPCs) cells. This has resulted in significant advances on the use of such expanded cells in transplantation settings. To this day, however, it is still unclear to what extent those stem and progenitor cells generated in vitro retain the functional and genomic integrity of their freshly isolated counterparts. In trying to contribute to the solving of this issue, in the present study we have selected and purified three different hematopoietic cell populations: HSCs (CD34⁺ CD38^- CD45RA^- CD71^- Lin^- cells), myeloid progenitor cells (CD34⁺ CD38⁺ CD45RA⁺ CD71^- L

SUBMITTER: Dircio-Maldonado R 

PROVIDER: S-EPMC6090508 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature