Linking genotype and phenotype in an economically viable propionic acid biosynthesis process.

ABSTRACT:

Background

Propionic acid (PA) is used as a food preservative and increasingly, as a precursor for the synthesis of monomers. PA is produced mainly through hydrocarboxylation of ethylene, also known as the 'oxo-process'; however, Propionibacterium species are promising biological PA producers natively producing PA as their main fermentation product. However, for fermentation to be competitive, a PA yield of at least 0.6 g/g is required.

Results

A new strain able to reach the required yield was obtained using genome shuffling. To gain insight into the changes leading to the improved phenotype, the genome of the new strain was sequenced, and metabolomics and transcriptomics data were obtained. In combination, the data revealed three key mutations: (i) a mutation in the

SUBMITTER: Luna-Flores CH 

PROVIDER: S-EPMC6090647 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature