A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle.

ABSTRACT:

Background

The 2014-2016 Ebola virus (EBOV) outbreak in West Africa highlighted the need for improved therapeutic options against this virus. Approaches targeting host factors/pathways essential for the virus are advantageous because they can potentially target a wide range of viruses, including newly emerging ones and because the development of resistance is less likely than when targeting the virus directly. However, systematic approaches for screening host factors important for EBOV have been hampered by the necessity to work with this virus at biosafety level 4 (BSL4).

Methods

In order to identify host factors involved in the EBOV life cycle, we performed a genome-wide siRNA screen comprising 64,755 individual siRNAs against 21,566 human genes to assess their activity in

SUBMITTER: Martin S 

PROVIDER: S-EPMC6090742 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature