Protocol for serum exosomal miRNAs analysis in prostate cancer patients treated with radiotherapy.

ABSTRACT:

Background

Circulating exosomes from prostate cancer (PCa) patients undergoing radiotherapy are attractive candidate biomarkers for monitoring treatment response. Multiple workflows for isolation and content characterization of exosomes in biofluids have been attempted. We report a protocol to isolate and characterize exosomal miRNAs content and assess radiation-induced changes.

Methods

In this pilot study, we performed targeted exosomal miRNA profiling of 25 serum samples obtained from PCa patients with intermediate- and high-risk disease treated with curative radiotherapy (RT), and controls. Post-treatment blood samples were collected at least 28 days after radiation therapy as a paired follow-up sample. The complete workflow consisted of two phases: I) filtration and poly

SUBMITTER: Malla B 

PROVIDER: S-EPMC6090775 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature