Validation of a simplex PCR assay enabling reliable identification of clinically relevant Candida species.

ABSTRACT:

Background

Fungal bloodstream infections (BSI) may be serious and are associated with drastic rise in mortality and health care costs. Candida spp. are the predominant etiological agent of fungal sepsis. The prompt and species-level identification of Candida may influence patient outcome and survival. The aim of this study was to develop and evaluate the CanTub-simplex PCR assay coupled with T_m calling and subsequent high resolution melting (HRM) analysis to barcode seven clinically relevant Candida species.

Methods

Efficiency, coefficient of correlation and the limit of reliable detection were estimated on purified Candida EDTA-whole blood (WB) reference panels seeded with Candida albicans, Candida glabrata, Candida parapsilosis, Candida tropicalis, Candida kruse

SUBMITTER: Fidler G 

PROVIDER: S-EPMC6090785 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature