DNA barcoding of blackflies (Diptera: Simuliidae) as a tool for species identification and detection of hidden diversity in the eastern regions of Spain.

ABSTRACT:

Background

Blackflies have negative impact on public and animal health due to the haematophagous habit of females. In recent times, in some regions in Spain, blackfly outbreaks are becoming more and more frequent, threatening the public health. However, there is still a paucity of data concerning the Spanish blackfly fauna. Correct identification of species is of paramount importance in order to provide correct information on species distribution, biology and behaviour, so that control measures could be implemented appropriately.

Methods

Blackflies specimens (larvae, pupae, reared adults and biting females) were collected in the period 2015-2017 in and near rivers and streams from different regions in Spain. A modified Hotshot technique was used for the DNA extraction and th

SUBMITTER: Ruiz-Arrondo I 

PROVIDER: S-EPMC6090827 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature