Characterization of TaDREB1 in wheat genotypes with different seed germination under osmotic stress.

ABSTRACT:

Background

The cis-acting element DRE/CRT plays an important role in activating gene expression responsive to osmotic stress, low temperature and high-salinity. DREB1/CBF genes encode DRE-binding proteins with the function as transcript activators. TaDREB1 was also found to be induced by osmotic stress.

Methods

The dates of osmotic stress was assessed by seed germination drought resistance index; the full-length cDNA sequences of TaDREB1 gene were downloaded from NCBI datebase; identification of allelic variation and transcript expression were assessed by PCR and semi-quantitive RT-PCR analysis, respectively.

Results

Total 13 new allele variations of TaDREB1 were identified in the germplasms tested in the paper, including 5

SUBMITTER: Liu M 

PROVIDER: S-EPMC6090928 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature