A facile in vitro platform to study cancer cell dormancy under hypoxic microenvironments using CoCl₂.

ABSTRACT:

Background

While hypoxia has been well-studied in various tumor microenvironments, its role in cancer cell dormancy is poorly understood, in part due to a lack of well-established in vitro and in vivo models. Hypoxic conditions under conventional hypoxia chambers are relatively unstable and cannot be maintained during characterization outside the chamber since normoxic response is quickly established. To address this challenge, we report a robust in vitro cancer dormancy model under a hypoxia-mimicking microenvironment using cobalt chloride (CoCl₂), a hypoxia-mimetic agent, which stabilizes hypoxia inducible factor 1-alpha (HIF1α), a major regulator of hypoxia signaling.

Methods

We compared cellular responses to CoCl₂ and true hypoxia (0.1% O₂

SUBMITTER: Lee HR 

PROVIDER: S-EPMC6091074 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature