DNA barcoding of the genus Nepenthes (Pitcher plant): a preliminary assessment towards its identification.

ABSTRACT:

Background

DNA barcoding is impending towards the generation of universal standards for species discrimination with a standard gene region that can be sequenced accurately and within short span of time. In this study, we were successful in developing efficient barcode locus in the Nepenthes genus. A total of 317 accessions were retrieved from GenBank of NCBI which represent 140 different species Nepenthes and evaluated the efficacy of ITS, rbcl and matK barcode candidates using barcode gap, applied distance similarity, and tree-based methods.

Result

Our result indicates that single-locus ITS or combined with plastid regions (matK) showed the best species discrimination with distinctive barcoding gaps. Therefore, we tentatively proposed the combination of ITS+matK as a core b

SUBMITTER: Gogoi B 

PROVIDER: S-EPMC6091102 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature