Isoliquiritigenin suppresses human melanoma growth by targeting miR-301b/LRIG1 signaling.

ABSTRACT:

Background

Isoliquiritigenin (ISL), a natural flavonoid isolated from the root of licorice (Glycyrrhiza uralensis), has shown various pharmacological properties including anti-oxidant, anti-inflammatory and anti-cancer activities. MicroRNAs (miRNAs), a class of small non-coding RNAs, have been reported as post-transcriptional regulators with altered expression levels in melanoma. This study aims to investigate the anti-melanoma effect of ISL and its potential mechanism.

Methods

We investigated the effect of ISL on the proliferation and apoptosis of melanoma cell lines with functional assays, such as CCK-8 assay, colony formation assay and flow cytometry. The protein level of apoptosis related genes were measured by western blotting. High-throughput genome sequencing was used

SUBMITTER: Xiang S 

PROVIDER: S-EPMC6091185 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature