Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

ABSTRACT:

Background

The sensory epithelium of the inner ear converts the mechanical energy of sound to electro-chemical energy recognized by the central nervous system. This process is mediated by receptor cells known as hair cells that express proteins in a timely fashion with the onset of hearing.

Methods

The proteomes of 3, 14, and 30 day-old mice cochlear sensory epithelia were revealed, using label-free quantitative mass spectrometry (LTQ-Orbitrap). Statistical analysis using a one-way ANOVA followed by Bonferroni's post-hoc test was used to show significant differences in protein expression. Ingenuity Pathway Analysis was used to observe networks of differentially expressed proteins, their biological processes, and associated diseases, while Cytoscape software was used t

SUBMITTER: Darville LNF 

PROVIDER: S-EPMC6091194 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature