Comparative functional dynamics studies on the enzyme nano-bio interface.

ABSTRACT:

Introduction

Biomedical applications of nanoparticles (NPs) as enzyme inhibitors have recently come to light. Oxides of metals native to the physiological environment (eg, Fe, Zn, Mg, etc.) are of particular interest-especially the functional consequences of their enzyme interaction.

Materials and methods

Here, Fe₂O₃, zinc oxide (ZnO), magnesium oxide (MgO) and nickel oxide (NiO) NPs are compared to copper (Cu) and boron carbide (B₄C) NPs. The functional impact of NP interaction to the model enzyme luciferase is determined by 2-dimensional fluorescence difference spectroscopy (2-D FDS) and 2-dimensional photoluminescence difference spectroscopy (2-D PLDS). By 2-D FDS analysis, the change in maximal intensity and in 2-D FDS area under the curv

SUBMITTER: Thomas SE 

PROVIDER: S-EPMC6092129 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature