Altered oral microbiota in chronic hepatitis B patients with different tongue coatings.

ABSTRACT:

Aim

To elucidate tongue coating microbiota and metabolic differences in chronic hepatitis B (CHB) patients with yellow or white tongue coatings.

Methods

Tongue coating samples were collected from 53 CHB patients (28 CHB yellow tongue coating patients and 25 CHB white tongue coating patients) and 22 healthy controls. Microbial DNA was extracted from the tongue samples, and the bacterial 16S ribosomal RNA gene V3 region was amplified from all samples and sequenced with the Ion Torrent PGM™ sequencing platform according to the standard protocols. The metabolites in the tongue coatings were evaluated using a liquid chromatography-mass spectrometry (LC-MS) platform. Statistical analyses were then performed.

Results

The relative compositions of the tongue coating microbiota

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC6092577 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature