RIM-binding proteins recruit BK-channels to presynaptic release sites adjacent to voltage-gated Ca²⁺-channels.

ABSTRACT: The active zone of presynaptic nerve terminals organizes the neurotransmitter release machinery, thereby enabling fast Ca²⁺-triggered synaptic vesicle exocytosis. BK-channels are Ca²⁺-activated large-conductance K⁺-channels that require close proximity to Ca²⁺-channels for activation and control Ca²⁺-triggered neurotransmitter release by accelerating membrane repolarization during action potential firing. How BK-channels are recruited to presynaptic Ca²⁺-channels, however, is unknown. Here, we show that RBPs (for RIM-binding proteins), which are evolutionarily conserved active zone proteins containing SH3- and FN3-domains, directly bind to BK-channels. We find that RBPs interact with RIMs and Ca²⁺-channels via the

SUBMITTER: Sclip A 

PROVIDER: S-EPMC6092624 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature