Hydrogen and thiosulfate limits for growth of a thermophilic, autotrophic Desulfurobacterium species from a deep-sea hydrothermal vent.

ABSTRACT: Hydrothermal fluids (341°C and 19°C) were collected < 1 m apart from a black smoker chimney and a tubeworm mound on the Boardwalk edifice at the Endeavour Segment in the northeastern Pacific Ocean to study anaerobic microbial growth in hydrothermal mineral deposits. Geochemical modelling of mixed vent fluid and seawater suggests the mixture was anoxic above 55°C and that low H2 concentrations (79 μmol kg(-1) in end-member hydrothermal fluid) limit anaerobic hydrogenotrophic growth above this temperature. A thermophilic, hydrogenotrophic sulfur reducer, Desulfurobacterium strain HR11, was isolated from the 19°C fluid raising questions about its H2 -dependent growth kinetics. Strain HR11 grew at 40-77°C (Topt 72-75°C), pH 5-8.5 (pHopt 6-7) and 1-5% (wt vol(-1) ) NaCl (NaClopt 3-4%). The high

SUBMITTER: Stewart LC 

PROVIDER: S-EPMC6092745 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature