Cell type-specific CLIP reveals that NOVA regulates cytoskeleton interactions in motoneurons.

ABSTRACT:

Background

Alternative RNA processing plays an essential role in shaping cell identity and connectivity in the central nervous system. This is believed to involve differential regulation of RNA processing in various cell types. However, in vivo study of cell type-specific post-transcriptional regulation has been a challenge. Here, we describe a sensitive and stringent method combining genetics and CLIP (crosslinking and immunoprecipitation) to globally identify regulatory interactions between NOVA and RNA in the mouse spinal cord motoneurons.

Results

We developed a means of undertaking motoneuron-specific CLIP to explore motoneuron-specific protein-RNA interactions relative to studies of the whole spinal cord in mouse. This allowed us to pinpoint differential RNA regulation

SUBMITTER: Yuan Y 

PROVIDER: S-EPMC6092797 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature