In-solution Y-chromosome capture-enrichment on ancient DNA libraries.

ABSTRACT:

Background

As most ancient biological samples have low levels of endogenous DNA, it is advantageous to enrich for specific genomic regions prior to sequencing. One approach-in-solution capture-enrichment-retrieves sequences of interest and reduces the fraction of microbial DNA. In this work, we implement a capture-enrichment approach targeting informative regions of the Y chromosome in six human archaeological remains excavated in the Caribbean and dated between 200 and 3000 years BP. We compare the recovery rate of Y-chromosome capture (YCC) alone, whole-genome capture followed by YCC (WGC + YCC) versus non-enriched (pre-capture) libraries.

Results

The six samples show different levels of initial endogenous content, with very low (< 0.05%, 4 samples) or low (0.1-1.54%, 2 sa

SUBMITTER: Cruz-Davalos DI 

PROVIDER: S-EPMC6092841 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature