PROMIS, global analysis of PROtein-metabolite interactions using size separation in Arabidopsis thaliana.

ABSTRACT: Small molecules not only represent cellular building blocks and metabolic intermediates, but also regulatory ligands and signaling molecules that interact with proteins. Although these interactions affect cellular metabolism, growth, and development, they have been largely understudied. Herein, we describe a method, which we named PROtein-Metabolite Interactions using Size separation (PROMIS), that allows simultaneous, global analysis of endogenous protein-small molecule and of protein-protein complexes. To this end, a cell-free native lysate from Arabidopsis thaliana cell cultures was fractionated by size-exclusion chromatography, followed by quantitative metabolomic and proteomic analyses. Proteins and small molecules showing similar elution behavior, a

SUBMITTER: Veyel D 

PROVIDER: S-EPMC6093232 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature