Induced pluripotent stem cell-based mapping of β-globin expression throughout human erythropoietic development.

ABSTRACT: Robust β-globin expression in erythroid cells derived from induced pluripotent stem cells (iPSCs) increases the resolution with which red blood cell disorders such as sickle cell disease and β thalassemia can be modeled in vitro. To better quantify efforts in augmenting β-globin expression, we report the creation of a β-globin reporter iPSC line that allows for the mapping of β-globin expression throughout human erythropoietic development in real time at single-cell resolution. Coupling this tool with single-cell RNA sequencing (scRNAseq) identified features that distinguish β-globin-expressing cells and allowed for the dissection of the developmental and maturational statuses of iPSC-derived erythroid lineage cells. Coexpression of embryonic, fetal, and adult globins in individual cells i

SUBMITTER: Vanuytsel K 

PROVIDER: S-EPMC6093724 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature