Inter-α inhibitor proteins maintain neutrophils in a resting state by regulating shape and reducing ROS production.

ABSTRACT: The plasma levels of inter-α inhibitor proteins (IAIPs) are decreased in patients with sepsis and the reduced levels correlate with increased mortality. In the present study, we examined the effects of IAIPs on human neutrophils to better understand the beneficial effects of IAIPs in the treatment of sepsis. We demonstrated that IAIPs induced a spherical shape that was smaller in size with a smooth cellular surface in a concentration-dependent manner. These changes were inhibited by a specific antibody against IAIPs. In contrast, bikunin, light chain of IAIP, had no effect on neutrophil morphology. The neutrophils treated with IAIPs could easily pass through the artificial microcapillaries and were prevented from entrapment inside the capillaries. Coincubation of human blood neutrophils wi

SUBMITTER: Htwe SS 

PROVIDER: S-EPMC6093744 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature