Unraveling the Plasmodium vivax sporozoite transcriptional journey from mosquito vector to human host.

ABSTRACT: Malaria parasites transmitted by mosquito bite are remarkably efficient in establishing human infections. The infection process requires roughly 30 minutes and is highly complex as quiescent sporozoites injected with mosquito saliva must be rapidly activated in the skin, migrate through the body, and infect the liver. This process is poorly understood for Plasmodium vivax due to low infectivity in the in vitro models. To study this skin-to-liver-stage of malaria, we used quantitative bioassays coupled with transcriptomics to evaluate parasite changes linked with mammalian microenvironmental factors. Our in vitro phenotyping and RNA-seq analyses revealed key microenvironmental relationships with distinct biological functions. Most notable, preservation of sporozoite quiescence by exposure t

SUBMITTER: Roth A 

PROVIDER: S-EPMC6093925 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature