Recapitulation of Human Neural Microenvironment Signatures in iPSC-Derived NPC 3D Differentiation.

ABSTRACT: Brain microenvironment plays an important role in neurodevelopment and pathology, where the extracellular matrix (ECM) and soluble factors modulate multiple cellular processes. Neural cell culture typically relies on heterologous matrices poorly resembling brain ECM. Here, we employed neurospheroids to address microenvironment remodeling during neural differentiation of human stem cells, without the confounding effects of exogenous matrices. Proteome and transcriptome dynamics revealed significant changes at cell membrane and ECM during 3D differentiation, diverging significantly from the 2D differentiation. Structural proteoglycans typical of brain ECM were enriched during 3D differentiation, in contrast to basement membrane constituents in 2D. Moreover, higher expression of synaptic and

SUBMITTER: Simao D 

PROVIDER: S-EPMC6094163 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature