Hypoxia promotes acquisition of aggressive phenotypes in human malignant mesothelioma.

ABSTRACT:

Background

Hypoxia is a hallmark of the solid tumor microenvironment and is associated with poor outcomes in cancer patients. The present study was performed to investigate mechanisms underlying the hypoxia-induced phenotypic changes using human malignant mesothelioma (HMM) cells.

Methods

Hypoxic conditions were achieved by incubating HMM cells in the air chamber. The effect of hypoxia on phenotype changes in HMM cells was investigated by performing in vitro clonogenicity, drug resistance, migration, and invasion assays. Signaling pathways and molecules involved in the more aggressive behaviors of HMM cells under hypoxia were investigated. A two-tailed unpaired Student's t-test or one-way ANOVA with Bonferroni post-test correction was used in this study.

Results

Hypox

SUBMITTER: Kim MC 

PROVIDER: S-EPMC6094475 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature