Molecular characterization of hepatitis A virus from children hospitalized at a tertiary care centre in northwest India.

ABSTRACT:

Background & objectives

Hepatitis A virus (HAV) infection is a major cause of childhood hepatitis, prevalent worldwide. HAV is classified into seven genotypes I-VII; genotypes III and I are the most common among humans. The present work was carried out to identify the genotypes prevalent in children suspected to have acute viral hepatitis (AVH), hospitalized at a tertiary care centre in northwest India.

Methods

A total of 1269 blood samples from children (0-15 yr of age) clinically suspected of viral hepatitis were screened for anti-HAV IgM. Acute phase serum was processed for RNA extraction and amplified by nested polymerase chain reaction (PCR) followed by sequencing of representative samples.

Results

Among the 1269 samples tested, 642 (50.59%) were positive for ant

SUBMITTER: Malhotra B 

PROVIDER: S-EPMC6094522 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature