Getting value from the waste: recombinant production of a sweet protein by Lactococcus lactis grown on cheese whey.

ABSTRACT: BACKGROUND:Recent biotechnological advancements have allowed for the adoption of Lactococcus lactis, a typical component of starter cultures used in food industry, as the host for the production of food-grade recombinant targets. Among several advantages, L. lactis has the important feature of growing on lactose, the main carbohydrate in milk and a majoritarian component of dairy wastes, such as cheese whey. RESULTS:We have used recombinant L. lactis NZ9000 carrying the nisin inducible pNZ8148 vector to produce MNEI, a small sweet protein derived from monellin, with potential for food industry applications as a high intensity sweetener. We have been able to sustain this production using a medium based on the cheese whey from the production of ricotta cheese, with minimal pre-treatment of t

SUBMITTER: Boumaiza M 

PROVIDER: S-EPMC6094915 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature