Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting.

ABSTRACT: Immunotherapies (IT) require induction, expansion, and maintenance of specific changes to a patient's immune cell repertoire which yield a therapeutic benefit. Recently, mechanistic understanding of these changes at the cellular level has revealed that IT results in complex phenotypic transitions in target cells, and that therapeutic effectiveness may be predicted by monitoring these transitions during therapy. However, monitoring will require unique tools that enable capture, manipulation, and profiling of rare immune cell populations. In this study, we introduce a method of automated and unsupervised separation and processing of rare immune cells, using high-force and multidimensional magnetic ratcheting (MR). We demonstrate capture of target immune cells using samples with up to 1 : 10 

SUBMITTER: Murray C 

PROVIDER: S-EPMC6095198 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature