A compact, low-cost, quantitative and multiplexed fluorescence detection platform for point-of-care applications.

ABSTRACT: An effective method of combating infectious diseases is the deployment of hand-held devices at the point-of-care (POC) for screening or self-monitoring applications. There is a need for very sensitive, low-cost and quantitative diagnostic devices. In this study, we present a low-cost, multiplexed fluorescence detection platform that has a high sensitivity and wide dynamic range. Our system features inexpensive 3 × 3 mm interference filters with a high stopband rejection, sharp transition edges, and greater than 90% transmission in the passband. In addition to the filters, we improve signal-to-noise ratio by leveraging time for accuracy using a charge-integration-based readout. The fluorescence sensing platform provides a sensitivity to photon flux of ∼1×10⁴photons/mm²

SUBMITTER: Obahiagbon U 

PROVIDER: S-EPMC6095205 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature