Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-Cell RNA Sequencing.

ABSTRACT: The definition of neuronal type and how it relates to the transcriptome are open questions. Drosophila olfactory projection neurons (PNs) are among the best-characterized neuronal types: different PN classes target dendrites to distinct olfactory glomeruli, while PNs of the same class exhibit indistinguishable anatomical and physiological properties. Using single-cell RNA sequencing, we comprehensively characterized the transcriptomes of most PN classes and unequivocally mapped transcriptomes to specific olfactory function for six classes. Transcriptomes of closely related PN classes exhibit the largest differences during circuit assembly but become indistinguishable in adults, suggesting that neuronal subtype diversity peaks during development. Transcription factors and cell-surface molec

SUBMITTER: Li H 

PROVIDER: S-EPMC6095479 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature