Retrograde Transport by Clathrin-Coated Vesicles is Involved in Intracellular Transport of PrP^Sc in Persistently Prion-Infected Cells.

ABSTRACT: Intracellular dynamics of an abnormal isoform of prion protein (PrP^Sc) are tightly associated with prion propagation. However, the machineries involved in the intracellular trafficking of PrP^Sc are not fully understood. Our previous study suggested that PrP^Sc in persistently prion-infected cells dynamically circulates between endocytic-recycling compartments (ERCs) and peripheral regions of the cells. To investigate these machineries, we focused on retrograde transport from endosomes to the trans-Golgi network, which is one of the pathways involved in recycling of molecules. PrP^Sc was co-localized with components of clathrin-coated vesicles (CCVs) as well as those of the retromer complex, which are known as machineries for retrograde transport. F

SUBMITTER: Yamasaki T 

PROVIDER: S-EPMC6095914 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature