Current approaches for the fitting and refinement of atomic models into cryo-EM maps using CCP-EM.

ABSTRACT: Recent advances in instrumentation and software have resulted in cryo-EM rapidly becoming the method of choice for structural biologists, especially for those studying the three-dimensional structures of very large macromolecular complexes. In this contribution, the tools available for macromolecular structure refinement into cryo-EM reconstructions that are available via CCP-EM are reviewed, specifically focusing on REFMAC5 and related tools. Whilst originally designed with a view to refinement against X-ray diffraction data, some of these tools have been able to be repurposed for cryo-EM owing to the same principles being applicable to refinement against cryo-EM maps. Since both techniques are used to elucidate macromolecular structures, tools encapsulating prior knowledge about macromol

SUBMITTER: Nicholls RA 

PROVIDER: S-EPMC6096485 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature