Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes.

ABSTRACT: The black yeast genus Exophiala includes numerous potential opportunistic species that potentially cause systematic and disseminated infections in immunocompetent individuals. Species causing systemic disease have ability to grow at 37-40 °C, while others consistently lack thermotolerance and are involved in diseases of cold-blooded, waterborne vertebrates and occasionally invertebrates. We explain a fast and sensitive assay for recognition and identification of waterborne Exophiala species without sequencing. The ITS rDNA region of seven Exophiala species (E. equina, E. salmonis, E. opportunistica, E. pisciphila, E. aquamarina, E. angulospora and E. castellanii) along with the close relative Veronaea botryosa was sequenced and aligned for the design of specific padlock probes for the dete

SUBMITTER: Najafzadeh MJ 

PROVIDER: S-EPMC6096892 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature