Induction and recovery of copy number variation in banana through gamma irradiation and low-coverage whole-genome sequencing.

ABSTRACT: Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to introduce novel alleles and broaden genetic diversity. We previously established a method for the induction and recovery of single nucleotide mutations generated with the chemical mutagen EMS. However, officially released mutant banana varieties have been created using gamma rays, a mutagen that can produce large genomic insertions and deletions (indels). Such dosage mutations may be important for generating observable phenotypes in polyploids. In this study, we establish a low-c

SUBMITTER: Datta S 

PROVIDER: S-EPMC6097122 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature