Trans-species synthetic gene design allows resistance pyramiding and broad-spectrum engineering of virus resistance in plants.

ABSTRACT: To infect plants, viruses rely heavily on their host's machinery. Plant genetic resistances based on host factor modifications can be found among existing natural variability and are widely used for some but not all crops. While biotechnology can supply for the lack of natural resistance alleles, new strategies need to be developed to increase resistance spectra and durability without impairing plant development. Here, we assess how the targeted allele modification of the Arabidopsis thaliana translation initiation factor eIF4E1 can lead to broad and efficient resistance to the major group of potyviruses. A synthetic Arabidopsis thaliana eIF4E1 allele was designed by introducing multiple amino acid changes associated with resistance to potyvirus in naturally occurring Pisum sativum alleles

SUBMITTER: Bastet A 

PROVIDER: S-EPMC6097130 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature